This SAE Recommended Practice describes the dynamic and static testing procedures required to evaluate the integrity of the ambulance substructure, to support the safe mounting of a SAE J3027 compliant litter retention device or system, when exposed to a frontal, side or rear impact (i.e. a crash impact). Its purpose is to provide manufacturers, ambulance builders, and end-users with testing procedures and, where appropriate, acceptance criteria that, to a great extent, ensure the ambulance substructure meets the same performance criteria across the industry. Prospective manufacturers or vendors have the option of performing either dynamic testing or static testing. Descriptions of the test set-up, test instrumentation, photographic/video coverage, test fixture, and performance metrics are included.